Peroxisomes are eukaryotic specific organelles that perform diverse metabolic functions including fatty acid β-oxidation, reactive species metabolism, photorespiration, and responses to stress.

The CRISPR-Cas9 system in bacteria and archaea has recently been exploited for genome editing in various model organisms, including mice. The CRISPR-Cas9 reagents can be delivered directly into the ...

The introduction of a targeted DNA break by designer endonucleases is a powerful technology that leverages evolutionarily conserved repair pathways to conduct genome engineering at the site of the ...

Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation.

In spite of being a very tractable and versatile technology, recombineering has limitations. For example, as very short regions of homology are sufficient for the recombination, manipulating regions ...

Tissues from rhesus monkeys were screened by PCR for the presence of sequences homologous to known adeno-associated virus (AAV) serotypes 1–6. DNA spanning entire rep-cap ORFs from two novel AAVs, ...