BioTechniques

Soil searching: new dPCR ‘DNA test’ roots out plants’ subterranean secrets

It’s a bit like giving the soil a DNA test.” A new droplet digital PCR (ddPCR) method quantifies root DNA directly from soil ...
GitHub

ddpcr: Analysis and visualization of Droplet Digital PCR data in R and on the web

This package provides an interface to explore, analyze, and visualize droplet digital PCR (ddPCR) data in R. It also includes an interactive web application with a visual user interface to facilitate ...
GEN

Adapting a Replication-Competent AAV Assay for Commercial Manufacturing

Adeno-associated virus (AAV) vectors play a pivotal role in gene therapy, delivering therapeutic genes with high precision and safety. However, a significant challenge in the biomanufacturing of these ...
C&EN

Introducing Theoretical Principles of Semi-, Relative, and Absolute Quantification via Conventional, Real-Time, and Digital PCR to Graduate and Senior Undergraduate Students of ...

Quantitative analysis in analytical chemistry is divided into semi-, relative, and absolute analysis. An example of each analysis is pH test paper, internal standard, and standard addition, ...
GEN

Cappable-seq: A Versatile Toolkit for the Identification of Transcriptional Landmarks in Bacteria

This method for directly enriching the 5´ end of primary transcripts enables the determination of transcription start sites at single-base resolution. Coupled with long read sequencing, the method ...
Nature

Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing

Solid-phase proximity ligation assays share properties with the classical sandwich immunoassays for protein detection. The proteins captured via antibodies on solid supports are, however, detected not ...